Product ID:Lysis Buffer A, Plant, Z919
MSDS Date:06/13/2002
MSDS Number: EEACY
Kit Part:=== Responsible Party ===
Company Name:Promega Corporation
Address:2800 Woods Hollow Road
City:Madison
State:WI
ZIP:53711
Country:US
Info Phone Num:1-800-356-9526, (608)-274-4330
Chemtrec Ind/Phone:1-800-424-9300

Telephone: 1-800-356-9526, (608)-274-4330

guanidinium thiocyanate

CAS: 593-84-0

Health Hazards Acute and Chronic:Hazard description Xn Harmful
Information pertaining to particular dangers for man and
environment The product has to be labelled due to the calculation
procedure of the "General Classification guideline for
preparations of the EU" in the latest valid version. R 20/21/22
Harmful by inhalation, in contact with skin and if swallowed.
NFPA ratings (scale 0-4) Health = 2 Fire = 0 Reactivity = 0 HMIS
Ratings Health = 2 Flammability = 0 Reactivity = 0
Medical Cond Aggravated by Exposure:

First Aid:General information Symptoms of poisoning may even occur
after several hours; therefore medical observation for at least 48
hours after the accident. After inhalation Supply fresh air. If
required, provide artificial respiration. Keep patient warm.
Consult doctor if symptoms persist. In case of unconsciousness
place patient stably in side position for transportation. After
skin contact Generally the product does not irritate the skin.
After eye contact Rinse opened eye for several minutes under
running water. After swallowing Immediately call a doctor.

Flash Point:Not applicable
Extinguishing Media:Suitable extinguishing agents CO2, extinguishing
powder or water spray. Fight larger fires with water spray or
alcohol resistant foam. Protective equipment: Mount respiratory
protective device.
Fire Fighting Procedures:No special measures required.
Unusual Fire/Explosion Hazard:Danger of explosion: Product does not
present an explosion hazard. Auto igniting: Product is not
selfigniting.

Spill Release Procedures:Person-related safety precautions: Not
required. Measures for environmental protection: Dilute with
plenty of water. Do not allow to enter sewers/surface or ground
water. Measures for cleaning/collecting: Absorb with
liquid-binding material (sand, diatomite, acid binders, universal
binders, sawdust). Dispose contaminated material as waste according
to item 13. Ensure adequate ventilation.
Neutralizing Agent:

Handling and Storage Precautions:Handling Information for safe
handling: Keep receptacles tightly sealed. Ensure good
ventilation/exhaustion at the workplace. Information about
protection against explosions and fires: No special measures
required. Storage Requirements to be met by storerooms and
receptacles: No special requirements. Information about storage
in one common storage facility: Do not store together with
oxidizing and acidic materials.
Other Precautions:Further information about storage conditions: Keep
receptacle tightly sealed. Storage class Class according to
regulation on flammable liquids: Not Applicable

Respiratory Protection:In case of brief exposure or low pollution use
respiratory filter device. In case of intensive or longer exposure
use respiratory protective device that is independent of
circulating air.
Protective Gloves:Protective gloves.
Eye Protection:Goggles recommended during refilling.
Work Hygienic Practices:Keep away from foodstuffs, beverages and feed.
Wash hands before breaks and at the end of work. Avoid contact
with the eyes and skin.
Supplemental Safety and Health

Boiling Pt:100 ° C
Melt/Freeze Pt:undetermined
Spec Gravity:at 20 ° C 1.102 g/cm³
Solubility in Water:Fully miscible
Appearance and Odor:Form: Fluid Color: Colorless Odor: Characteristic
Corrosion Rate:

Stability Indicator/Materials to Avoid:Stability Condition to
Avoid:Reacts with acids, alkalis and oxidizing agents
Hazardous Decomposition Products:No decomposition if used according to
specifications. Dangerous products of decomposition: Carbon
monoxide and carbon dioxide Nitrogen oxides (NOx) Sulfur oxides
(SOx)
Conditions to Avoid Polymerization:

Waste Disposal Methods:Product: Recommendation Must not be disposed
of together with household garbage. Do not allow product to reach
sewage system. Uncleaned packagings: Recommendation: Disposal
must be made according to official regulations. Recommended
cleansing agent: Water, if necessary with cleansing agents.

Toxicological Information:Acute toxicity: Primary irritant effect: on
the skin: Irritant to skin and mucous membranes. on the eye:
Irritating effect. Sensitization: No sensitizing effects known.
Additional toxicological information: The product shows the
following dangers according to the calculation method of the
General EU Classification Guidelines for Preparations as issued in
the latest version: Harmful

Disclaimer (provided with this information by the compiling agencies):
This information is formulated for use by elements of the Department
of Defense. The United States of America in no manner whatsoever,
expressly or implied, warrants this information to be accurate and
disclaims all liability for its use. Any person utilizing this
document should seek competent professional advice to verify and
assume responsibility for the suitability of this information to their
particular situation.

Ecological:General notes: Water hazard class 1 (Self-assessment):
slightly hazardous for water. Do not allow undiluted product or
large quantities of it to reach ground water, water course or
sewage system.

Transport Information:Contact Promega Safety Department for additional
transportation information DOT regulations: Hazard class: -
Land transport ADR/RID (cross-border) ADR/RID class: -
Maritime transport IMDG: IMDG Class: - Marine pollutant: No
Air transport ICAO-TI and IATA-DGR: ICAO/IATA Class: -

SARA Title III Information:Section 355 (Extremely hazardous
substance): None of the ingredients is listed. Section 313
(Specific toxic chemical listings): None of the ingredients is
listed.
Federal Regulatory Information:TSCA (Toxic Substance Control Act):
All ingredients are listed. Proposition 65 Chemicals known to
cause cancer: None of the ingredients is listed. Chemicals
known to cause reproductive toxicity: None of the ingredients
is listed. Cancerogenity categories EPA (Environmental Protection
Agency) None of the ingredients is listed. IARC (International
Agency for Research on Cancer) None of the ingredients is
listed. NTP (National Toxicology Program) None of the
ingredients is listed. TLV (Threshold Limit Value established by
ACGIH) None of the ingredients is listed. MAK (German Maximum
Workplace Concentration) None of the ingredients is listed.
NIOSH-Ca (National Institute for Occupational Safety and Health)
None of the ingredients is listed. OSHA-Ca (Occupational Safety &
Health Administration) None of the ingredients is listed.
State Regulatory Information: